Exhibit 23.01
Consent of Independent Registered Public Accounting Firm
We consent to the incorporation by reference in Registration Statements (Nos. 333-107442,
333-85242, 333-73244, 333-47980, 333-87791) on Form S-8 of our reports dated December 11, 2009,
relating to the consolidated financial statements of Keynote Systems, Inc. and subsidiaries (the
Company) and the effectiveness of the Companys internal control over financial reporting (which
report on the consolidated financial statements includes an explanatory paragraph relating to the
Companys adoption of ASC 740-10, Accounting for Uncertainty in Income Taxes an interpretation of FASB Statement No. 109),
appearing in the Annual Report on Form 10-K of Keynote Systems, Inc. for the year ended
September 30, 2009.
/s/ DELOITTE & TOUCHE LLP
San Jose, California
December 11, 2009